Supporting Document 1 Application A1046 – Food Derived from Herbicide- Tolerant Soybean Line Das-68416-4 Safety Assessment Report

Dow AgroSciences Australia Ltd has developed a genetically modified (GM) soybean line known as DAS-68416-4 that is tolerant to two herbicides, glufosinate ammonium and 2,4-dichlorophenoxyacetic acid (2,4-D). Tolerance to glufosinate ammonium is achieved through expression of the enzyme phosphinothricin acetyl transferase (PAT) encoded by the pat gene derived from the soil bacterium Streptomyces viridochromogenes. The pat and closely related bar genes have been widely used in the genetic modification of a number of crop species. Tolerance to 2,4-D has been achieved through the introduction of the aad-12 gene, from another soil bacterium Delftia acidovorans, expressing the enzyme aryloxyalkanoate dioxygenase-12 (AAD-12).